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pet28 expression vector  (Millipore)

 
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    Millipore pet28 expression vector
    Pet28 Expression Vector, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet28 expression vector/product/Millipore
    Average 90 stars, based on 1 article reviews
    pet28 expression vector - by Bioz Stars, 2026-03
    90/100 stars

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    SDS–PAGE for induced expression of recombinant proteins (αA-crystallin-pet28, βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.

    Journal: Molecular Vision

    Article Title: The expression of αA- and βB1-crystallin during normal development and regeneration, and proteomic analysis for the regenerating lens in Xenopus laevis

    doi:

    Figure Lengend Snippet: SDS–PAGE for induced expression of recombinant proteins (αA-crystallin-pet28, βB1-crystallin-pet28) and western blotting analysis for the two specific antibodies. Recombinant proteins were expressed in E. coli Rosetta (DE3). Their respective molecular weigh are 19 kDa and 23 kDa. In western blotting analysis, sample 1, 2, and 3 are total lens proteins; samples 4 and 5 are purified fusion proteins. By results, good specificity is shown.

    Article Snippet: The resulting 555 bp and 741 bp fragments were digested with Nco I and Hind III (Takara, Tokyo, Japan) at 37 °C for 5 h, cloned into pET28 expression vectors (Invitrogen) digested by Nco I and Hind III (Nco I and Psc I are isocaudarners).

    Techniques: SDS Page, Expressing, Recombinant, Western Blot, Purification